| 货号 | 86050S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | T-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, COX-2, cytochrome c, GADD45α, and HIF-1α (8-13). |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
Western blot analysis of extracts from various cell lines uisng TIA-1 (D1Q3K) Rabbit mAb. | |
Immunoprecipitation of TIA-1 from KARPAS-299 cell lysates. Lane 1 represents 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TIA-1 (D1Q3K) Rabbit mAb. Western blot was performed using TIA-1 (D1Q3K) Rabbit mAb. A conformation specific secondary antibody was used to avoid cross-reactivity with IgG. Cell line source: Dr. Abraham Karpas at the University of Cambridge. |
