| 货号 | 76012S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-F |
| 目标/特异性 | mGluR2 (D7D8M) Rabbit mAb recognizes endogenous levels of total mGluR2 protein. This antibody may cross-react with mGluR3. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Metabotropic glutamate receptor 2 (mGluR2) is a class C G protein-coupled receptor for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). While mGluR1 and mGluR5 activate phospholipase C, mGluR2, mGluR3, mGluR4, and mGluR6 are coupled to the inhibitory G protein Gα(i/o) and inhibit adenylyl cyclase (AC) activity (1). Research studies have suggested that mGluR2/3 receptors may be potential targets for the treatment of Schizophrenia (2). Furthermore, mGluR2 interacts with the 5HT2A serotonin receptor to form a hetero-complex in the brain. This complex is a potential pharmacological target for hallucinogenic drugs (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 100, >200 |
| 参考文献 | 1 . Pin, J.P. et al. (1994) EMBO J 13, 342-8. 2 . Patil, S.T. et al. (2007) Nat Med 13, 1102-7. 3 . González-Maeso, J. et al. (2008) Nature 452, 93-7. 4 . González-Maeso, J. and Sealfon, S.C. (2009) Trends Neurosci 32, 225-32. |
Western blot analysis of extracts from various tissues using mGluR2 (D7D8M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of mGluR2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is mGluR2 (D7D8M) Rabbit mAb. Western blot analysis was performed using mGluR2 (D7D8M) Rabbit mAb. | |
Confocal immunofluorescent analysis of rat hippocampus (left) and cerebellum (right) using mGluR2 (D7D8M) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
