| 货号 | 4775S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | Tid-1 (RS13) Mouse Monoclonal Antibody detects endogenous levels of the short and long variants of Tid-1. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Human Tid-1 is a human orthologue of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l (2) tid and is a member of the DnaJ family of proteins that serve as co-chaperones to Hsp70 proteins (1). These proteins are characterized by a J domain, a highly conserved tetrahelical domain that binds to Hsp70 chaperones and activates their ATPase activity. Hsp70 and their associated chaperones mediate a variety of activities including the folding of newly synthesized polypeptides, the translocation of proteins across membranes and assembly of multimeric protein complexes. Two alternatively spliced variants exist for human Tid-1 ,designated hTID-1s and hTID-1L, both which contain the J domain, localize to the mitochondrial matrix, and co-immunoprecipitate with Hsp70. Expression of Tid-1L increases apoptosis induced by the DNA damaging agent mitomycin c (MMC) and by TNF-alpha, and that activity is dependent on its J domain. In contrast, expression of Tid-1S reduces apoptosis by these agents. Tid-1 orthologues are also found in mouse (mTid-1) and rat (rTid-1) (2,3). The mouse orthologue was originally identified though its interaction with p120 GTPase-activating protein (GAP), raising the possiblity that Tid-1 helps regulates the confirmation, activity, or subcellular localization of GAP (3). |
| 存放说明 | -20C |
| 计算分子量 | 37 Tid-1s. 40 Tid-1L. |
Western blot analysis of extracts from Jurkat (human), C6 (rat), and L929 (mouse) cells, using Tid-1 (RS13) Mouse mAb. 对Jurkat（人）、C6（大鼠）和L929（小鼠）细胞提取物使用Tid-1 (RS13) Mouse mAb进行Western blot分析。 |
