| 货号 | 13377S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from various cell lines using TNF-R1 (D3I7K) Rabbit mAb (Rodent Specific). | |
Western blot analysis of extracts from L-929 cells, transfected with 100 nm SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TNF-R1 siRNA I (Mouse Specific) #13369 (+), or SignalSilence® TNF-R1 siRNA II (Mouse Specific) #13570 (+), using TNF-R1 (D3I7K) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The TNF-R1 (D3I7K) Rabbit mAb (Rodent Specific) confirms the silencing of the TNF-R1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse TNF-R1 (mTNF-R1; +), using TNF-R1 (D3I7K) Rabbit mAb (Rodent Specific). |
