TNF-α (D5G9) Rabbit mAb【科瑞奥博】

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TNF-α (D5G9) Rabbit mAb

货号： 6945S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	6945S
反应种属	Human
来源宿主	Rabbit
应用	W/IP
目标/特异性	TNF-α (D5G9) Rabbit mAb recognizes endogenous levels of total full length and secreted TNF-α protein. It can detect up to 10 pg of recombinant TNF-α by western blot.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
背景	TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease (1,2,4).
存放说明	-20C
计算分子量	18, 25

参考图片

Western blot analysis of recombinant human TNF-α #8902 using TNF-α (D5G9) Rabbit mAb (left) or TNF-α Antibody #3707 (right).Western免疫印迹。用TNF-α (D5G9) Rabbit mAb (左图)或TNF-α Antibody #3707 (右图) 研究融合的人源TNF-α #8902 蛋白。

Western blot analysis of extracts from the media of THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; overnight), using TNF-α (D5G9) Rabbit mAb.Western免疫印迹。用TNF-α (D5G9) Rabbit mAb研究经 TPA #4174 (80 nM; 过夜)处理的, 然后未经处理和经 LPS (1 μg/ml; 过夜)处理的血管中层THP-1细胞的细胞提取液。

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; various time points), using TNF-α (D5G9) Rabbit mAb.Western免疫印迹。用TNF-α (D5G9) Rabbit mAb研究经 TPA #4174 (80 nM; 过夜)处理的, 然后未经处理和经 LPS (1 μg/ml; 过夜)处理的THP-1细胞的细胞提取液。