Toll-like Receptor 2 (D7G9Z) Rabbit mAb【科瑞奥博】

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Toll-like Receptor 2 (D7G9Z) Rabbit mAb

货号： 12276S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12276S
反应种属	Human
来源宿主	Rabbit
应用	W/IP
目标/特异性	Toll-like Receptor 2 (D7G9Z) Rabbit mAb recognizes endogenous levels of total TLR2 protein.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
背景	Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.Toll样受体(TLR) 的家族成员, 是以与其密切相关的果蝇Toll受体而命名的，TLR在先天免疫中发挥了极其重要的作用(1-3)。TLRs 能够识别不同病原菌中保守的结构域并介导防御应答。TLR的触发导致了NF-κB信号通路的激活并因此调控下游的免疫和炎症基因的表达。TLRs与IL-1受体家族都有一个大约200个氨基酸的保守区域叫做TIR域。被激活后, TLRs与细胞质中的含有TIR区域的一系列接头蛋白作用，包括MyD88 (骨髓分化因子), MAL/TIRAP (MyD88-adaptor-like/TIR-associated 蛋白), TRIF (Toll-receptor相关的干扰素激活物)和 TRAM (Toll-receptor相关的分子).。这些反应导致了IRAK1 和 IRAK4的招募和激活并与TRAF6 形成复合体激活 TAK1 和 IKK。IKK的激活导致了因锚定NF-κB在细胞质中而使其失去活性的IκB的降解。
存放说明	-20C
计算分子量	90-105

参考图片

Western blot analysis of THP-1 and MUTZ-3 cells using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.Western blot 分析THP-1和MUTZ-3细胞的细胞提取物，使用抗体是Toll-like Receptor 2 (D7G9Z) Rabbit mAb。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR2 (hTLR2-HA; +), using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.Western blot分析293T细胞的细胞提取物，对照转染(-)或转染表达HA标签全长人源TLR2的构建质粒(hTLR2-HA; +), 使用抗体是Toll-like Receptor 2 (D7G9Z) Rabbit mAb。

Immunoprecipitation of TLR2 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 2 (D7G9Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.对MUTZ-3细胞中的TLR2进行免疫沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 Toll-like Receptor 2 (D7G9Z) Rabbit mAb (泳道3)。泳道1为10%的加入量。Western blot分析使用抗体是 Toll-like Receptor 2 (D7G9Z) Rabbit mAb。