Toll-like Receptor 3 (D10F10) Rabbit mAb【科瑞奥博】

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Toll-like Receptor 3 (D10F10) Rabbit mAb

货号： 6961S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	6961S
反应种属	Human
来源宿主	Rabbit
应用	W
目标/特异性	Toll-like Receptor 3 (D10F10) Rabbit mAb recognizes endogenous levels of total TLR3 protein. A band is detected at 75 kDa in some cell lines/tissues which is of unknown origin.
使用方法	WB(1:1000)

性能
供应商	CST
背景	Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm.
存放说明	-20C
计算分子量	115-130

参考图片

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR3 (+), using Toll-like Receptor 3 (D10F10) Rabbit mAb.Western疫印迹。用Toll-like Receptor 3 (D10F10) Rabbit mAb研究对照转染和转染了人源TLR3 (+)的293T 细胞的细胞提取液。

Western blot analysis of extracts from HT-29 cells, untreated or following transfection with pIpC (100 μg/ml; overnight), using Toll-like Receptor 3 (D10F10) Rabbit mAb.Western疫印迹。用Toll-like Receptor 3 (D10F10) Rabbit mAb研究未经处理和转染了pIpC (100 μg/ml; 过夜)的HT-29 细胞的细胞提取液。

Western blot analysis of extracts from MCF 10A cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Toll-like Receptor 3 siRNA I #6236 (+), using Toll-like Receptor 3 (D10F10) Rabbit mAb #6961 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Toll-like Receptor 3 (D10F10) Rabbit mAb confirms silencing of Toll-like Receptor 3 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western疫印迹。用Toll-like Receptor 3 (D10F10) Rabbit mAb #6961 (上图) 或α-Tubulin (11H10) Rabbit mAb #2125 (下图)研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® Toll-like Receptor 3 siRNA I #6236 (+)的MCF 10A细胞的细胞提取液。 Toll-like Receptor 3 (D10F10) Rabbit mAb 证实了对Toll-like Receptor 3表达的沉默，而α-Tubulin (11H10) Rabbit mAb 作为上样量的对照。