| 货号 | 5632S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Toll-like Receptor 7 (D7) Rabbit mAb detects endogenous levels of total human TLR7 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from various cell lines using Toll-like Receptor 7 (D7) Rabbit mAb.Western免疫印迹。用Toll-like Receptor 7 (D7) Rabbit mAb研究各种细胞的细胞提取液。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR7 (+), using Toll-like Receptor 7 (D7) Rabbit mAb.Western疫印迹。用Toll-like Receptor 7 (D7) Rabbit mAb研究对照转染(-) 和转染了人源TLR7 (+)的293T 细胞的细胞提取液。 |
