Toll-like Receptor 8 (D3Z6J) Rabbit mAb
货号：
11886S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 11886S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TLR8 (D3Z6J) Rabbit mAb recognizes endogenous levels of total TLR8 protein. This antibody cross-reacts with a 30 kDa protein and a 37 kDa protein of unknown origin. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.Toll样受体（TLR）家族成员是对果蝇中密切相关的一系列Toll样受体的命名，它们在先天免疫反应中发挥着举足轻重的作用（1-4）。TLRs能识别各种病原体中的保守基序和调解防御反应（5-7）。TLR通路的触发往往导致NF-κB的活化及随后的免疫和炎症基因的调控（4）。TLRs和白介素1受体家族成员共享一个约200个氨基酸的保守区域，称为Toll/IL-1受体（TIR）结构域（1）。激活后，TLRs与许多细胞质（包含TIR结构域）的接头蛋白相关，包括髓样分化因子88（MyD88）、MyD88样接头蛋白/TIR结构域接头蛋白（MAL/TIRAP）、Toll受体相关的干扰素活化剂（TRIF）和Toll受体相关的分子（TRAM）（8-10）。这种关联导致IRAK1和IRAK4的补充和活化，能与TRAF6形成复合物激活TAK1和IKK（8,11-14）。IKK的活化能导致IκB的降解，通常情况下，IκB使NF-κB以失活状态存在于细胞质中。 |
| 存放说明 | -20C |
| 计算分子量 | 150 |
参考图片Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, 16 hr), untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (20 ng/mL, 8 hr; +), using TLR8 (D3Z6J) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析TPA #4174诱导分化(80 nM, 16 小时)的THP-1细胞的提取物，未处理(-)或Human Interferon-γ (hIFN-γ) #8901 (20 ng/mL, 8小时; +)处理。使用的抗体是：TLR8 (D3Z6J) Rabbit mAb（上图）、β-Actin (D6A8) Rabbit mAb #8457（下图）。 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR8 (hTLR8-HA; +), using TLR8 (D3Z6J) Rabbit mAb.Western blot分析293T细胞的提取物，对照转染(-)或用HA标记的全长的人TLR8(hTLR8-HA; +)转染。使用的抗体是：TLR8 (D3Z6J) Rabbit mAb。 |
