| 货号 | 14342S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Topoisomerase II Binding Protein 1 (TopBP1) contains eight BRCT domains, which facilitate interaction with various proteins, phosphopeptides, and DNA. Through these interactions, TopBP1 functions in the regulation of DNA replication, DNA repair, checkpoint control, and transcription (1). TopBP1 contacts the checkpoint kinase ATR and its binding partner ATRIP, and induces ATR and Chk1 activation in collaboration with claspin (2,3). Activation of ATR is dependent on recruitment of TopBP1 through the MRN (MRE11-RAD50-NBS1) complex, and the 911 (RAD9-RAD1-HUS1) complex is required for full activation (4). TopBP1 stabilizes Bloom syndrome helicase (BLM) during S phase of the cell cycle to suppress sister chromatin exchange and maintain genome stability (5). |
| 存放说明 | -20C |
| 计算分子量 | 170 |
Western blot analysis of extracts from various cell types using TopBP1 (D8G4L) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb (lower) #6487. | |
Immunoprecipitation of TopBP1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TopBP1 (D8G4L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using TopBP1 (D8G4L) Rabbit mAb. |
