| 货号 | 12339S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TPH-1 (D10C10) Rabbit mAb recognizes endogenous levels of total TPH-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin (4). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human TPH-1 (hTPH-1; +) and P815 cells, using TPH-1 (D10C10) Rabbit mAb (upper) or α/β-Tubulin Antibody #2148 (lower). Western blot方法检测293T细胞提取物：空质粒转染（-）或全长人TPH-1 (hTPH-1; +)或P815细胞转染；使用的抗体是TPH-1 (D10C10) Rabbit mAb兔单抗（上图）或α/β-Tubulin Antibody 兔多抗#2148（下图）。 |
