| 货号 | 41658S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. |
| 存放说明 | -20C |
| 计算分子量 | 64 |
Western blot analysis of extracts from various cell lines using TRAF5 (D3E2R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TRAF5 (hTRAF5-Myc/DDK; +) using TRAF5 (D3E2R) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Western blot analysis of extracts from A172 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TRAF5 siRNA I #60131 (+), or SignalSilence® TRAF5 siRNA II #38805 using TRAF5 (D3E2R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The TRAF5 (D3E2R) Rabbit mAb confirms silencing of TRAF5 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. |
