| 货号 | 3219S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | TRAIL (C92B9) Rabbit mAb detects endogenous levels of total human TRAIL protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) F (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (10-13) and DcR2 (TRAIL-R4, TRUNDD) (14-15) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (16). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (17-18). |
| 存放说明 | -20C |
| 计算分子量 | 28-30 |
Flow cytometric analysis of 786-0 cells using TRAIL (C92B9) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞仪检测786-0细胞，使用抗体为TRAIL (C92B9) Rabbit mAb 兔单抗（蓝色），一个非特异性阴性对照抗体（红色）作为对照。 |
