| 货号 | 13387S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TRIM33 (E1N2Z) Rabbit mAb recognizes endogenous levels of total TRIM33 protein. Based upon sequence alignment, this antibody is predicted to react with TRIM33 isoforms A and B, but not with other TIF family members. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | The transcriptional intermediary factor 1 (TIF1) family represents a group of proteins with multiple histone-binding domains. In humans, this family comprises four proteins, TIF1α/TRIM24, TIF1β/TRIM28/KAP1, TIF1γ/TRIM33/Ectodermin, and TIF1δ/TRIM66, which are characterized by an amino-terminal tripartite motif (TRIM) domain consisting of a RING domain, two B boxes, a coiled-coil domain, and a carboxy-terminal PHD finger and bromodomain (1). Despite their similar overall structure, these proteins have diverse roles in transcriptional regulation. TIF1α functions as a ligand-dependent nuclear receptor coregulator and more recently has been implicated in regulating p53 stability (2). TIF1β is an intrinsic component of the N-CoR1 corepressor complex and the NuRD nucleosome-remodeling complex (3) and functions as a corepressor for Kruppel-associated box (KRAB) zinc-finger transcription factors (4). Furthermore, TIF1β promotes heterochromatin-mediated gene silencing formation by serving as a cofactor for heterochromatin protein HP1 (5). TIF1δ expression is restricted to the testis and has been shown to interact with HP1γ (6). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
Western blot analysis of extracts from various cell lines using TRIM33 (E1N2Z) Rabbit mAb. 使用TRIM33 (E1N2Z) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TRIM33 siRNA I #13487 (+), or SignalSilence® siRNA II #13503 (+), using TRIM33 (E1N2Z) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The TRIM33 (E1N2Z) Rabbit mAb confirms silencing of TRIM33, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. 使用TRIM33 (E1N2Z) Rabbit mAb兔单抗（上）或GAPDH (D16H11) XP® Rabbit mAb #5174（下）对转染了100 nM SignalSilence® Control siRNA (未偶联) #6568 (-), SignalSilence® TRIM33 siRNA I (+), 或 SignalSilence® TRIM33 siRNA II #12440 (+)的293T细胞提取物进行western blot分析。TRIM33 (E1N2Z) Rabbit mAb用于确认TRIM33被敲除，GAPDH (D16H11) XP® Rabbit mAb用于确认上样量一致。 |
