| 货号 | 82481S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | RASAL2 (D6K9L) Rabbit mAb recognizes endogenous levels of total RASAL2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Small GTPases act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. RASAL2 was initially identified as a GAP for the small GTPase, Ras, and later shown to interact with the Rho-GEF ECT2, and to regulate Rho activity in human astrocytoma cells (1). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Weeks, A. et al. (2012) Am J Pathol 181, 662-74. 2 . McLaughlin, S.K. et al. (2013) Cancer Cell 24, 365-78. 3 . Huang, Y. et al. (2014) Oncotarget 5, 6734-45. 4 . Li, N. and Li, S. (2014) Biochem Biophys Res Commun 455, 358-62. 5 . Feng, M. et al. (2014) J Clin Invest 124, 5291-304. |
Immunoprecipitation of RASAL2 from MDA-MB-231 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is RASAL2 (D6K9L) Rabbit mAb. Western blot analysis was performed using | |
Western blot analysis of extracts from HeLa and MDA-MB-231 cells using RASAL2 (D6K9L) Rabbit mAb. |
