| 货号 | 4607S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IHC-P/F |
| 目标/特异性 | TrkB (80G2) Rabbit mAb detects endogenous levels of total TrkB protein. The antibody does not cross-react with TrkA. |
| 使用方法 | IHC-P(1:80) F (1:200) |
| 供应商 | CST |
| 背景 | The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).The phosphorylation sites are conserved between TrkA and TrkB: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674/675 of TrkA to Tyr706/707 in TrkB of the human sequence (7). TrkB is overexpressed in tumors such as neuroblastoma, prostate adenocarcinoma and pancreatic ductal adenocarcinoma. In neuroblastomas overexpression of TrkB correlates with unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms’s tumors and this isoform may act as a dominant-negative to TrkB signaling (8). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Immunohistochemical analysis of paraffin-embedded NIH/3T3/TrkB (left) or NIH/3T3/TrkA (right) cell pellets using TrkB (80G2) Rabbit mAb. 免疫组织化学分析石蜡包埋的NIH/3T3/TrkB（左图）或NIH/3T3/TrkA(右图)细胞团块，使用的抗体是TrkB(80G2) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TrkB (80G2) Rabbit mAb. 免疫组化分析石蜡包埋的人肺癌切片，使用的抗体是TrkB(80G2)Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of NIH/3T3 cells, untransfected (red) or TrkB transfected (blue), using TrkB (80G2) Rabbit mAb. 流式细胞分析NIH/3T3细胞，未转染（红色）或经TrkB转染（蓝色），使用的抗体是TrkB (80G2) Rabbit mAb兔单抗。 |
