Tuberin/TSC2 (28A7) Rabbit mAb【科瑞奥博】

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Tuberin/TSC2 (28A7) Rabbit mAb

货号： 3635S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	3635S
反应种属	Human
来源宿主	Rabbit
应用	W/IP
目标/特异性	Tuberin/TSC2 (28A7) Rabbit mAb detects endogenous levels of total tuberin.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
背景	Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).
存放说明	-20C
计算分子量	200

参考图片

Western blot analysis of extracts from control SH-SY5Y, differentiated SH-SY5Y, PC3 and LNCaP cells, using Tuberin/TSC2 (28A7) Rabbit mAb.

应用Tuberin/TSC2 (28A7) Rabbit 单克隆抗体，Western blot 分析SH-SY5Y对照，分化的SH-SY5Y， PC3 和LNCaP等细胞提取物。

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Tuberin/TSC2 siRNA I (+) or SignalSilence® Tuberin/TSC2 siRNA II (+), using Tuberin/TSC2 (28A7) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Tuberin/TSC2 (28A7) Rabbit mAb confirms silencing of Tuberin/TSC2 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Tuberin/TSC2 siRNA.

Western blot分析Hela细胞提取物，采用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Tuberin/TSC2 siRNA I (+) 或 SignalSilence® Tuberin/TSC2 siRNA II (+)转染，所用抗体为Tuberin/TSC2 (28A7) Rabbit mAb兔单抗 and α-Tubulin (11H10) Rabbit mAb兔单抗 #2125.Tuberin/TSC2 (28A7) Rabbit mAb兔单抗确定了Tuberin/TSC2 表达的沉默，α-Tubulin (11H10) Rabbit mAb 作为上样和Tuberin/TSC2 siRNA的特异性的对照。