| 货号 | 3990S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Tuberin/TSC2 (D57A9) Rabbit mAb detects endogenous levels of total tuberin protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
Western blot analysis of extracts from SH-SY5Y and C6 cells, and from wild-type (wt) and TSC2-deficient (-/-) mouse embryonic fibroblasts (MEF), using Tuberin/TSC2 (D57A9) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). Western blot 分析SH-SY5Y 和C6 cells细胞提取物，来自于野生型（wt）和TSC2缺陷性（-/-）型小鼠的 胚胎fibroblasts (MEF)，所使用的抗体是Tuberin/TSC2 (D57A9) Rabbit mAb兔单抗（上）和Akt (pan) (C67E7) Rabbit mAb兔单抗 #4691 （下） 。 |
