UBE2S (D5H9H) Rabbit mAb【科瑞奥博】

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UBE2S (D5H9H) Rabbit mAb

货号： 11878S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	11878S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP
目标/特异性	UBE2S (D5H9H) Rabbit mAb recognizes endogenous levels of total UBE2S protein.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
背景	Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).
存放说明	-20C
计算分子量	26

参考图片

Western blot analysis of extracts from various cell lines using UBE2S (D5H9H) Rabbit mAb.Western blot检测多种细胞提取物。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2S (hUBE2S-Myc/DDK; +), using UBE2S (D5H9H) Rabbit mAb.Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged人全长UBE2S (hUBE2S-Myc/DDK; +)的293T细胞，采用UBE2S (D5H9H) Rabbit mAb。

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), or SignalSilence® UBE2S siRNA Ι #7220 (+), using UBE2S (D5H9H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The UBE2S (D5H9H) Rabbit mAb confirms silencing of UBE2S expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.Western blot检测转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® UBE2S siRNA Ι #7220 (+)的293T细胞，采用UBE2S (D5H9H) Rabbit mAb (upper)或GAPDH (D16H11) XP® Rabbit mAb #5174 (lower)。UBE2S (D5H9H) Rabbit mAb证实了UBE2S的沉默表达，采用GAPDH (D16H11) XP® Rabbit mAb作为内参照。

Immunoprecipitation of UBE2S from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or UBE2S (D5H9H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE2S (D5H9H) Rabbit mAb.Immunoprecipitation检测293T细胞提取物中的UBE2S，采用Rabbit (DA1E) mAb IgG XP®同型对照#3900 (lane 2) 或UBE2S (D5H9H) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%。Western blot检测采用UBE2S (D5H9H) Rabbit mAb。