UBE2T (D2L7H) Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

UBE2T (D2L7H) Rabbit mAb

货号： 12992S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12992S
反应种属	Human
来源宿主	Rabbit
应用	W/IP
目标/特异性	UBE2T (D2L7H) Rabbit mAb recognizes endogenous levels of total UBE2T protein.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
背景	Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).
存放说明	-20C
计算分子量	25

参考图片

Western blot analysis of extracts from various cell lines using UBE2T (D2L7H) Rabbit mAb.Western blot检测多种细胞提取物，使用的抗体是UBE2T (D2L7H) Rabbit mAb。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2T (hUBE2T-Myc/DDK; +), using UBE2T (D2L7H) Rabbit mAb.Western blot分析293T细胞提取物，对照未转染（-）或转染了Myc/DDK标签的全长人源的UBE2T (hUBE2T-Myc/DDK; +)，使用的抗体是UBE2T (D2L7H) Rabbit mAb。

Immunoprecipitation of UBE2T from 293T cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or UBE2T (D2L7H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE2T (D2L7H) Rabbit mAb.对293细胞提取物中的UBE2T进行免疫共沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或UBE2T (D2L7H) Rabbit mAb (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是UBE2T (D2L7H) Rabbit mAb。

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-) or SignalSilence^® UBE2T siRNA I #13142 (+), using UBE2T (D2L7H) Rabbit mAb (upper) or GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower). The UBE2T (D2L7H) Rabbit mAb confirms silencing of UBE2T expression, while the GAPDH (D16H11) XP^® Rabbit mAb is used as a loading control.Western blot检测293T细胞提取物，转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® UBE2T siRNA I #13142 (+)，使用的抗体是UBE2T (D2L7H) Rabbit mAb (上图)或GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。UBE2T (D2L7H) Rabbit mAb证实了UBE2T沉默表达，采用GAPDH (D16H11) XP® Rabbit mAb做对照。