| 货号 | 85813S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | PCSK9 (D7U6L) Rabbit mAb recognizes endogenous levels of total PCSK9 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3). |
| 存放说明 | -20C |
| 计算分子量 | 65, 80 |
| 参考文献 | 1 . Scamuffa, N. et al. (2006) FASEB J 20, 1954-63. 2 . Fugère, M. and Day, R. (2005) Trends Pharmacol Sci 26, 294-301. 3 . Seidah, N.G. and Chrétien, M. (1999) Brain Res 848, 45-62. 4 . Abifadel, M. et al. (2003) Nat Genet 34, 154-6. 5 . Cariou, B. et al. (2011) Atherosclerosis 216, 258-65. 6 . Cohen, J.C. et al. (2006) N Engl J Med 354, 1264-72. |
Immunoprecipitation of PCSK9 from T84 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PCSK9 (D7U6L) Rabbit mAb. Western blot analysis was performed using PCSK9 (D7U6L) Rabbit mAb. | |
Western blot analysis of extracts from T84 cells, untreated (-) or treated with Brefeldin A #9972 (100 ng/ml, 16 hrs; +), using PCSK9 (D7U6L) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
