| 货号 | 12863S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Western blot analysis of extracts from U-937 cells, untreated (-) or treated with TPA #4174 (200 nM, 72 hr; +), using uPAR (D4Q5S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).未处理(-)或经过TPA #4174 (200 nM, 72 hr; +)处理的U-937细胞提取物，使用uPAR (D4Q5S) Rabbit mAb (上) 或 β-Actin (D6A8) Rabbit mAb #8457 (下)进行western blot分析。 | |
Immunoprecipitation of uPAR from U-937 cell extracts treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (200 nM, 72 hr; +) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or uPAR (D4Q5S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using uPAR (D4Q5S) Rabbit mAb as the primary antibody and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 as the secondary antibody.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 uPAR (D4Q5S) Rabbit mAb (lane 3)对经过TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (200 nM, 72 hr; +)处理的U-937细胞中的uPAR进行免疫沉淀实验。Lane1是10%上样量。uPAR (D4Q5S) Rabbit mAb作为一抗，Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678作为二抗进行western blot分析。 |
