| 货号 | 12713S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 35-65 |
Confocal immunofluorescent analysis of HT-1080 cells using uPAR (D7X2N) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用uPAR (D7X2N) Rabbit mAb(绿色)对HT-1080细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from U-937 cells, untreated (-) or treated (+) with TPA #4174 (200 nM, 72 hr), using uPAR (D7X2N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).未处理(-)或经过TPA #4174 (200 nM, 72 hr)处理(+)的U-937细胞提取物，使用uPAR (D7X2N) Rabbit mAb (上) 或 β-Actin (D6A8) Rabbit mAb #8457 (下)进行western blot分析。 |
