| 货号 | 12040S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Upf1 (D15G6) Rabbit mAb recognizes endogenous levels of total Upf1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Confocal immunofluorescent anlaysis of Huh7 cells, untreated (left) or heat shock-treated (right), using Upf1 (D15G6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术检测经过热激（左）和未经过热激（右）的Huh7细胞、使用的抗体为Upf1 (D15G6) Rabbit mAb 兔单抗(绿色)。蓝色伪彩=DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from various cell lines using Upf1 (D15G6) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为Upf1 (D15G6) Rabbit mAb兔单抗。 | |
