| 货号 | 4970S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:200) IF-IC (1:200) IHC-F (1:50) |
| 供应商 | CST |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Immunohistochemical analysis of paraffin-embedded human leiomyoma using beta-Actin (13E5) Rabbit mAb.使用beta-Actin (13E5) Rabbit mAb，免疫组化（Immunohistochemical）分析人源平滑肌瘤石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human heart using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of cardiac actin.使用beta-Actin (13E5) Rabbit mAb，免疫组化（Immunohistochemical）分析人源心脏组织石蜡切片。说明在心脏actin的染色是缺少的。 | |
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of skeletal muscle actin.使用beta-Actin (13E5) Rabbit mAb，免疫组化（Immunohistochemical）分析人源骨骼肌石蜡切片。说明在骨骼肌actin的染色是缺少的。 | |
Western blot analysis of cell extracts from various cell lines using beta-Actin (13E5) Rabbit mAb.使用beta-Actin (13E5) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞系β-Actin蛋白水平。 | |
Flow cytometric analysis of NIH/3T3 cells using β-Actin (13E5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).与一个非特异阴性control antibody（红色）比较，使用β-Actin (13E5) Rabbit mAb（蓝色）标记，流式细胞仪分析NIH/3T3细胞。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using beta-Actin (13E5) Rabbit mAb in the presence of control peptide (left) or beta-Actin Blocking Peptide #1025 (right).使用beta-Actin (13E5) Rabbit mAb，免疫组化（Immunohistochemical）分析人源肺癌组织石蜡切片。其分别孵育control peptide (左图)和beta-Actin Blocking Peptide #1025 (右图)。 | |
Immunohistochemical analysis of frozen H1650 xenograft showing membrane and cytoplasmic localization using β-Actin (13E5) Rabbit mAb.使用β-Actin (13E5) Rabbit mAb，免疫组化（Immunohistochemical）分析H1650 xenograft冰冻切片。结果显示在膜和细胞质上的定位。 | |
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using β-actin (13E5) Rabbit mAb.使用beta-Actin (13E5) Rabbit mAb，免疫组化（Immunohistochemical）分析4T1 syngeneic mouse tumor组织石蜡切片。 | |
Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用β-Actin (13E5) Rabbit mAb（绿色）标记，共聚焦免疫荧光分析COS-7细胞。蓝色= DRAQ5® #4084（DNA荧光染料）。 |
