| 货号 | 13202S |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:800) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
Flow cytometric analysis of COS-7 cells, untransfected (blue) or transfected with a V5-tagged construct (green), using V5-Tag (D3H8Q) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of COS-7 cells, mock transfected (left) or transfected with a V5-tagged construct (right) using V5-Tag (D3H8Q) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with V5-tag fusion protein (+), using V5-Tag (D3H8Q) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457. | |
Immunoprecipitation of transfected V5-tag fusion protein from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or V5-Tag (D3H8Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using V5-Tag (D3H8Q) Rabbit mAb. | |
Confocal immunofluorescent analysis of COS -7 cells, mock transfected (right) or transfected with a V5-tagged construct (left) using V5-Tag (D3H8Q) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
