| 货号 | 8146S |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:800) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
Western blot analysis of extracts from 293T cells, untransfected or transfected with either DYKDDDDK-tagged FoxG1, NPAS4, or Tyrosine Hydroxylase (TH), using DYKDDDDK Tag (9A3) Mouse mAb.Western blot分析293T细胞提取物，未转染或者用DYKDDDDK标记的FoxG1，NPAS4或酪氨酸羟化酶（TH）转染。使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb。 | |
Confocal immunofluorescent analysis of HeLa cells transfected with a DYKDDDDK-tagged IRF3 construct using DYKDDDDK Tag (9A3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析用DYKDDDDK标记的IRF3质粒转染的HeLa细胞，使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb（绿色）。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left) or ROS-DYKDDDDK Tag transfected (right), using DYKDDDDK Tag (9A3) Mouse mAb.免疫组化分析石蜡包埋的293T细胞沉淀，对照（左图）或者ROS-DYKDDDDK标签转染的（右图），使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb。 | |
Flow cytometric analysis of Cos cells, untreated (blue) or transfected with a DYKDDDDK-tagged IRF3 contruct (green) using DYKDDDDK Tag (9A3) Mouse mAb.流式细胞检测分析Cos细胞，未处理（蓝色）或用DYKDDDDK标记的IRF3质粒转染（绿色），使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb。 |
