| 货号 | 2118S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein. |
| 使用方法 | WB(1:1000) IHC-P (1:800) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimers diseases (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb. 对多个细胞系使用GAPDH (14C10) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (14C10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人乳腺癌使用GAPDH (14C10) Rabbit mAb兔单抗进行免疫组织化学分析，其中左图为存在对照肽，右图为存在抗原特异性肽。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using GAPDH (14C10) Rabbit mAb. 对石蜡包埋的人前列腺癌使用GAPDH (14C10) Rabbit mAb兔单抗进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb antibody (blue) compared to a nonspecific negative control antibody (red). 使用GAPDH (14C10) Rabbit mAb antibody兔单抗（蓝）和非特异的阴性对照抗体（红），对HeLa细胞进行流式细胞仪分析。 | |
Confocal immunofluorescent analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用GAPDH (14C10) Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor? 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 |
