| 货号 | 2367S |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IHC-P (1:1200) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).免疫组化分析石蜡包埋的COS细胞，左图为未转染组，中图为HA-Akt3 转染组，右图为HA-雌激素受体转染组，所用抗体为Akt抗体（上图），雌激素受体抗体（中图）和HA-Tag (6E2) Mouse mAb (下图). | |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Akt3 (green), using HA-Tag (6E2) Mouse mAb compared to a nonspecific negative control antibody (red).流式细胞术分析COS细胞。蓝色为未转染组，绿色为用HA-Akt3转染组，所用抗体为HA-Tag (6E2) Mouse mAb ，与非特异性阴性对照抗体（红色）作比较。 | |
Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb.Weatern blot分析COS细胞提取物，泳道1 为未转染组和泳道2为表达HA-tagged Akt3组，泳道3为HA-tagged 雌激素受体(ER)， 所用抗体为HA-Tag (6E2) Mouse mAb。 | |
Immunocytochemical staining of COS cells without transfection (left), expressing HA-tagged Akt3 (middle) or expressing HA-tagged Estrogen Receptor (right), using HA-Tag (6E2) Mouse mAb.免疫细胞化学分析COS细胞，左图为未转染组，中图为表达HA-tagged Akt3 组，右图为表达雌激素受体组，所用抗体为HA-Tag (6E2) Mouse mAb。 | |
Confocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦显微镜分析COS细胞，左图为mock转染组，右图为Ha标签蛋白转染组，所用抗体为HA-Tag (6E2) Mouse mAb (绿色)。蓝色伪彩为荧光DNA燃料，产品信息为DRAQ5 ® #4084 。 |
