HA-Tag (C29F4) Rabbit mAb【科瑞奥博】

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HA-Tag (C29F4) Rabbit mAb

货号： 3724S 基本售价： 3250.0 元规格： 100ul

产品信息

概述
货号	3724S
反应种属	All
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC/F/ChIP
使用方法	WB(1:1000) IP (1:50) IHC-P (1:1600) F (1:1600) IF-IC (1:1600) ChIP (1:50)

性能
供应商	CST
背景	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties.
存放说明	-20C

参考图片

Flow cytometric analysis of HeLa cells, untransfected (blue) or transfected with HA-FoxO4 (green), using HA-Tag (C29F4) Rabbit mAb #3724.流式细胞术分析HeLa细胞，蓝色为未转染组，绿色为HA-FoxO4 转染组，所用抗体为HA-Tag (C29F4) Rabbit mAb #3724。

Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right) or HA-Tag transfected (left), using HA-Tag (C29F4) Rabbit mAb.免疫组化分析石蜡包埋的COS细胞，下图为未转染组，上图为HA-Tag转染组，所用抗体为HA-Tag (C29F4) Rabbit mAb。

Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb.Weatern blot分析HeLa细胞提取物，未转染组和采用 HA-FoxO4 或 HA-Akt3转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb。

Confocal immunofluorescent analysis of COS cells, transfected with an HA-tagged protein (left) or mock-transfected (right), using HA-Tag (C29F4) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).免疫荧光分析COS-7细胞提取物。左图为采用HA标签蛋白转染组，下图为mock转染组，所用抗体为HA-Tag (C29F4) Rabbit （绿色），肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084 。

293T cells were either untransfected (left panel) or transfected with an HA-tagged human CBP construct (right panel), then treated with Forskolin #3828 (30 µM) . Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ cells and 10 µl of HA-Tag (C29F4) Rabbit mAb, 10 µl of CBP (D9B6) Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP^® Human NR4A3 Promoter Primers #4829, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.