| 货号 | 2276S |
| 反应种属 | All |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) IP (1:1000) IHC-P (1:51200) F (1:500) IF-IC (1:8000) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
Western blot analysis of cell extracts expressing carboxy-terminal Myc-tagged protein (lane 1), amino-terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag (9B11) Mouse mAb.Western blot fenix细胞提取物羧基末端表达Myc-tagged 蛋白（泳道1），氨基末端表达Myc-tagged 蛋白（泳道2）和对照组提取物（泳道3），所用抗体为Myc-Tag (9B11) Mouse mAb。 | |
Immunoprecipitation of cell extracts alone (lane 1), extracts overexpressing carboxy-terminal Myc-tagged protein (lane 2) or amino-terminal Myc-tagged protein (lane 3), using Myc-Tag (9B11) Mouse mAb.免疫沉淀分析细胞提取物（泳道1），提取物羧基末端表达Myc-tagged 蛋白（泳道2）或氨基末端表达Myc-tagged 蛋白。所用抗体为 Myc-Tag (9B11) Mouse mAb。 | |
Flow cytometric analysis of COS cells, untreated (green) or Myc transfected (blue), using Myc-Tag (9B11) Mouse mAb.流式细胞术分选COS细胞，绿色为未转染组，蓝色为Myc转染的细胞，所用抗体为using Myc-Tag (9B11) Mouse mAb。 | |
Immunohistochemical analysis of paraffin-embedded COS cell pellets, control (left) or transfected with a carboxy-terminal Myc tagged protein (right), using Myc-Tag (9B11) Mouse mAb.免疫组化分析石蜡包埋的COS细胞小球，左图为对照组，右图为羧基末端带有Myc标签蛋白的转染组（右图），所用抗体为Myc-Tag (9B11) Mouse mAb。 | |
Confocal immunofluorescent analysis of Cos cells transfected with a Myc-tagged protein (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
293T cells were either untransfected (left panel) or transfected with an Myc-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 5 µl of Myc-Tag (9B11) Mouse mAb #2276, 5µl of Stat3α (D1A5) XP® Rabbit mAb #8768, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
