| 货号 | 9681S |
| 反应种属 | Human/Mouse/Rat/Monkey/All |
| 来源宿主 | Mouse |
| 应用 | W/E-P |
| 目标/特异性 | Acetylated-Lysine Mouse mAb (Ac-K-103) detects proteins only when posttranslationally modified by acetylation on the epsilon-amine groups of lysine residues. Detection of acetylated lysine by this antibody is largely independent of surrounding amino acid sequence. The antibody has been shown to recognize acetylated proteins including histones, p53, CBP, PCAF and chemically acetylated BSA. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10). |
| 存放说明 | -20C |
Western blot analysis of extracts from COS cells, untreated or TSA-treated (0.4 µM for 18 hours), using Acetylated-Lysine Mouse mAb (Ac-K-103). 对COS细胞，未处理或0.4uM TSA处理18小时，使用Acetylated-Lysine Mouse mAb (Ac-K-103)进行Western blot分析。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated (lane 2) or treated in vitro with CPB (lane 3) or PCAF (lane 4), using Acetylated-Lysine Mouse mAb (Ac-K-103). (Lanes 1 and 5: PCAF and CBP, respectively, showing auto acetylation. 对NIH/3T3细胞，未处理（列2）或体外CPB（列3）或PCAF（列4）处理，使用Acetylated-Lysine Mouse mAb (Ac-K-103)进行Western blot分析。（列1和列5：PCAF和CBP，显示自身乙酰化） |
