| 货号 | 46387S |
| 来源宿主 | Mouse |
| 应用 | W |
| 供应商 | CST |
| 背景 | Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. |
| 存放说明 | -20C |
Titration of Prestained Protein Marker, Broad Range (11-190 kDa) #13953 on a 4-20% Tris-glycine gradient SDS-polyacrylamide gel (left). Western Blot analysis of #13953 using Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) (right). | |
Titration of Pierce™ Prestained Protein MW markers [ThermoFisher #26612] on a 4-20% Tris-glycine gradient SDS-polyacrylamide gel (left). Western Blot analysis of Pierce™ Prestained Protein MW markers [ThermoFisher #26612] using Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) (right). | |
Titration of PageRuler™ Prestained Protein ladder, 10 to 180 kDa [ThermoFisher #26616] on a 4-20% Tris-glycine gradient SDS-polyacrylamide gel (left). Western Blot analysis of PageRuler™ Prestained Protein ladder, 10 to 180 kDa [ThermoFisher #26616] using Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) (right). |
