| 货号 | 9386S |
| 反应种属 | All |
| 来源宿主 | Mouse |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-Threonine (42H4) Mouse mAb binds phosphorylated threonine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific but does not cross-react with phospho-tyrosine-containing sequences. It does show slight cross-reactivity with a few phospho-serine-containing peptides. By ELISA, it recognizes a wide variety of threonine-phosphorylated peptides. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:50) E-P (1:2000) |
| 供应商 | CST |
| 背景 | Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior. General protein modification antibodies are designed to react with modified amino acid residues (e.g., phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs. |
| 存放说明 | -20C |
Western blot analysis of extracts from A431 cells, untreated or treated with calyculin A, a threonine phosphatase inhibitor, or extracts from NIH/3T3 cells, untreated or treated with pervanadate, a tyrosine phosphatase inhibitor, using Phospho-Threonine (42H4) Mouse mAb (upper) or Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) #9411 (lower). 对A431细胞抽提液，未处理（-）或calyculin A（苏氨酸磷酸酶抑制剂）处理，或者对NIH/3T3细胞抽提液，未处理或过钒酸盐（酪氨酸磷酸酶抑制剂）处理，使用Phospho-Threonine (42H4) Mouse mAb (上图)或Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) #9411 (下图)进行Western blot分析。 | |
Phospho-Threonine (42H4) Mouse mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* denotes phosphorylated threonine.) Phospho-Threonine (42H4) Mouse mAb ELISA分析：磷酸化和非磷酸化的信噪比。（T*代表磷酸化的苏氨酸） |
