| 货号 | 9417S |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) is prepared by biotinylation of the antibody via primary amines. Biotinylated antibody is useful for detection or purification of tyrosine-phosphorylated proteins using avidin-biotin binding. This antibody does not cross-react with proteins phosphorylated at serine or threonine. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:2000) IP (1:50) IHC-P (1:800) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from NIH/3T3 cells untreated and treated with sodium vanadate (1 mM for 30 minutes), using 75 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) (A), then detected by Anti-biotin Antibody, HRP-linked #7075. Western blot analysis of the same extract using 750 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411, then detected by Anti-mouse IgG, HRP-linked Antibody #7076 (B). 对NIH/3T3细胞系抽提物，未处理或钒酸钠（1mM，30分钟）处理，使用75 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) (A)进行Western blot分析，然后用Anti-biotin Antibody, HRP-linked #7075进行检测。对同一抽提物，使用750 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411进行Western blot分析，然后用Anti-mouse IgG, HRP-linked Antibody #7076 (B)进行检测。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or λ-phosphatase-treated (right), using Phospho-Tyrosine mAb (P-Tyr-100) (Biotinylated). 对石蜡包埋的人乳腺癌，未处理（左）或λ磷酸酶处理（右），使用Phospho-Tyrosine mAb (P-Tyr-100) (Biotinylated)进行免疫组化分析。 |
