| 货号 | 5625T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 89 |
| 参考文献 | 1 . Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 2 . Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 3 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 4 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. 5 . Tewari, M. et al. (1995) Cell 81, 801-809. 6 . Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539. |
共聚焦免疫荧光分析HeLa细胞，未处理（左）和Staurosporine #9953（右）处理，使用抗体为Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb兔单抗（绿色），DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5 ®＃4084（荧光DNA染料）。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (right), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (green). Actin filament were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). |
