Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb

货号： 4176T 基本售价： 1750.0 元规格： -

产品信息

概述
货号	4176T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IP , IF-F , IF-IC
目标/特异性	Phospho-β-Catenin (Ser675) (D2F1) XP^® Rabbit mAb detects endogenous levels of β-catenin only when phosphorylated at Ser675.
使用方法	WB(1:1000) IP (1:200) IF-F (1:100) IF-IC (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
存放说明	-20C
计算分子量	92
参考文献	1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. 9 . Hino S et al. (2005) Mol Cell Biol 25, 9063–72 10 . Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976.

参考图片

Confocal immunofluorescent analysis of rat colon using Phospho-β-Catenin (Ser675) (D2F1) XP^® Rabbit mAb (green). Actin filaments have been labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

使用Phospho-β-Catenin (Ser675) (D2F1) XP^® Rabbit mAb (绿色)对大鼠结肠组织进行激光共聚焦荧光分析. 肌动蛋白丝使用DY-554 Phalloidin（红色）标记。蓝色伪彩=DRAQ5^® #4084 (DNA荧光染料).

Western blot analysis of extracts from MKN-45 and SK-N-MC cells, untreated or treated with λ phosphatase for 1 hour or forskolin (FSK) for 30 minutes, using Phospho-β-Catenin (Ser675) (D2F1) XP^® Rabbit mAb (upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).

MKN-45 and SK-N-MC 细胞，未处理或经λ磷酸酶处理1小时或用福斯科林（FSK）处理30分钟，获得细胞提取物，使用Phospho-β-Catenin (Ser675) (D2F1) XP^® Rabbit mAb(上) 或β-Catenin (6B3) Rabbit mAb #9582 (下)进行western blot分析。

Confocal immunofluorescent analysis of HeLa cells, untreated (left), λ phosphatase-treated (middle), or untreated NCI-H28 cells (β-catenin null; right) using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

对未处理（左）或λ-磷酸酶处理（中）的HeLa细胞，或未经处理的NCI-H28细胞（β-catenin 缺失；右）使用Phospho-β-Catenin (Ser675) (D2F1) XP^® Rabbit mAb (绿色)进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554phalloidin（红色）标记.蓝色伪彩=DRAQ5^® #4084 (DNA荧光染料).