Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb
货号：
13448T 基本售价：
1750.0 元 规格：
20ul
产品信息
概述| 货号 | 13448T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb recognizes endogenous levels of lamin A/C protein only when phosphorylated at Ser22. |
| 使用方法 | WB(1:1000)
IP (1:100)
IF-IC (1:2500) |
性能| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 69,78 |
| 参考文献 | 1 . Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2 . Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3 . Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4 . Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5 . Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6 . Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
7 . Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5.
8 . Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
9 . Molina, H. et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204.
10 . Beausoleil, S.A. et al. (2006) Nat Biotechnol 24, 1285-92.
11 . Nousiainen, M. et al. (2006) Proc Natl Acad Sci U S A 103, 5391-6.
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参考图片Confocal immunofluorescent analysis of HeLa cells, untreated (left) or hydroxyurea-treated (right), using Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, treated with either hydroxyurea (4 mM, 20 hr; +) to induce G1/S phase or Paclitaxel #9807 (100 nM, 20 hr; +) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb (upper) and Lamin A/C Antibody #2032 (lower). |
Immunoprecipitation of phospho-Lamin A/C (Ser22) from extracts of HeLa cells treated with Paclitaxel #9807, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Lamin A/C (Ser22) (D2B2E) X® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb. |
