| 货号 | 4370T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Hamster,Monkey,Mink,D. melanogaster,Zebrafish,Bovine,Dog,Pig,S. cerevisiae, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases. |
| 使用方法 | WB(1:2000) IP (1:50) IHC-P (1:400) F (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 44, 42 |
| 参考文献 | 1 . Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. 2 . Baccarini, M. (2005) FEBS Lett 579, 3271-7. 3 . Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39. 4 . Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. 5 . Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74. 6 . Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75. 7 . Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. 8 . Marais, R. et al. (1993) Cell 73, 381-93. 9 . Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24. 10 . Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13. |
流式方法检测细胞提取物：U0126处理的(蓝色)、PMA处理的(绿色)Jurkat细胞，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb兔单抗。 | |
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb. | |
免疫组织化学方法检测石蜡包埋的人乳腺癌组织。使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb兔单抗。 | |
免疫组织化学方法检测石蜡包埋的人肺癌组织，左侧样品未经处理，右侧样品经λ磷酸酶处理。使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb. | |
免疫组织化学方法检测SignalSlide™ Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls #8103（石蜡包埋的NIH/3T3细胞，左侧为经U0126 #9903处理，右侧为经TPA #4174 处理）。使用的抗体为中的Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb on SignalSlide™ Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls #8103 (paraffin-embedded NIH/3T3 cells, treated with U0126 #9903 (left) or TPA #4174 (right). | |
Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (upper), or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower). |
