| 货号 | 8592T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , F , IF-IC |
| 目标/特异性 | Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43). |
| 使用方法 | WB(1:1000) IP (1:100) F (1:800) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. |
| 存放说明 | -20C |
| 计算分子量 | 18, 43 |
| 参考文献 | 1 . Muzio, M. et al. (1996) Cell 85, 817-27. 2 . Boldin, M.P. et al. (1996) Cell 85, 803-15. 3 . Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9. |
Confocal immunofluorescent analysis of Raw 264.7 cells using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析Raw 264.7细胞，使用抗体为Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) 兔单抗（绿色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Flow cytometric analysis of CTLL-2 cells, untreated (red) or treated with cyclohexamide and hTNF-α #8902 (blue), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific). 流式细胞仪检测CTLL-2细胞，未处理 (-)或hTNF-α #8902（蓝色）和 cyclohexamide (放线菌酮)处理，使用抗体为Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific)兔单抗。 | |
Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by hTNF-α #8902 (20 ng/ml, 4 hours), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific). Western blot 检测CTLL-2细胞提取物，未处理 (-)或 cycloheximide （放线菌酮）(CHX, 10 μg/ml, 过夜)处理，随后用hTNF-α #8902(20 ng/ml, 4 hr)处理后，用使用抗体为Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific)兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct overexpressing mouse caspase-8 (mCasp8) (+), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific). Western blot方法检测293T细胞提取物，空质粒（-）或过表达的小鼠caspase-8 (mCasp8)（+）载体转染；使用抗体为 Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific)兔单抗。 |
