| 货号 | 19807T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,Zebrafish,Dog, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-F , IF-IC |
| 目标/特异性 | Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb recognizes endogenous levels of β-catenin protein only when Ser45 is not phosphorylated. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1000) F (1:200) IF-F (1:1600) IF-IC (1:1600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. 9 . Sakanaka, C. (2002) J Biochem 132, 697-703. |
Western blot analysis of extracts from various cell lines using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of SW480 (left and lower right; positive) or NCI-H28 (upper right; negative) cells using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (green) in the presence of phospho-β-Catenin (Ser45) peptide (lower left) or non-phospho β-Catenin peptide (lower right). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of mouse colon using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of non-phospho β-catenin (Ser45) protein from COLO 205 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. Western blot analysis was performed using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb in the presence of phospho-β-Catenin (Ser45) peptide (left) or non-phospho β-Catenin (Ser45) peptide (right). | |
Immunohistochemical analysis of paraffin-embedded HeLa (left) or NCI-H28 (right) cell pellets using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. | |
Flow cytometric analysis of SW480 (green) and NCI-H28 (blue) cells using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
