| 货号 | 3270T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,Pig, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC , ChIP |
| 目标/特异性 | Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb detects endogenous levels of c-Jun only when phosphorylated at Ser73. This antibody may also recognize JunD phosphorylated at Ser100. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:200) IF-IC (1:800) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Jochum, W. et al. (2001) Oncogene 20, 2401-12. 2 . Davis, R.J. (2000) Cell 103, 239-52. 3 . Hilberg, F. et al. (1993) Nature 365, 179-81. 4 . Raivich, G. et al. (2004) Neuron 43, 57-67. 5 . Behrens, A. et al. (2002) EMBO J 21, 1782-90. 6 . Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700. 7 . Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. 8 . Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. 9 . Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3. 10 . Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20. 11 . Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. 12 . Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4. |
Flow cytometric analysis of HeLa cells, untreated (blue) or UV treated (green), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb.Flow cytometric方法检测细胞提取物：未经处理（蓝色）和紫外照射的（绿色）HeLa细胞，使用的抗体是Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，使用的抗体为Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。 | |
Immunohistochemical analysis of parafin-embedded human colon carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser73) Blocking Peptide (right).免疫组织化学方法检测石蜡包埋的人结肠癌组织，使用的抗体为Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人乳腺癌组织，左侧为对照组，右侧为lambda 磷酸酶处理的。使用的抗体为Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测：未经处理的（左侧）或茴香霉素处理的（右侧）HeLa 细胞，使用的抗体是 Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (绿色)，肌动蛋白纤维素已被 DY-554鬼笔环肽(红色)标记。 | |
Western blot analysis of extracts from NIH/3T3 or C6 cells, untreated or UV-treated, using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).Western blot方法检测细胞提取物：未经处理的和紫外照射的NIH/3T3、C6细胞。使用的抗体是Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb (上图)和c-Jun (60A8) Rabbit mAb #9165 (下图)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (hβ-NGF) #5221 (50 ng/ml) for 2h and either 10 μl of Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® using Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
