| 货号 | 2985S |
| 描述 | This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. |
| 来源宿主 | Rabbit IgG |
| 应用 | IF-IC/F |
| 使用方法 | IF-IC () |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851, untreated (blue) or IFN-α-treated (green).流式细胞术分析Jurkat细胞，所用抗体为Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor ® 647 Conjugate) (红色) 与Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb (Alexa Fluor ® 647 Conjugate) #4851作比较, 蓝色为未处理组，绿色为IFN-α处理组。 | |
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4075, untreated (green) or LY294002- and wortmannin-treated (blue).流式细胞术分析Jurkat细胞，所用抗体为Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor ® 647 Conjugate) (红色) 与Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb (Alexa Fluor ® 647 Conjugate) #4075作比较, 绿色为未处理组，蓝色为LY294002- 和 wortmannin处理组。 | |
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate) #3907 (pseudocolor blue, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate (pseudocolor blue, right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087 (fluorescent DNA dye). |
