Rabbit (DA1E) mAb IgG XP® Isotype Control【科瑞奥博】

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Rabbit (DA1E) mAb IgG XP® Isotype Control

货号： 3900S 基本售价： 1646.0 元规格： 250ug

产品信息

概述
货号	3900S
来源宿主	Rabbit IgG
应用	IP/IHC-P/IF-IC/F/ChIP
使用方法	IHC-P () F () IF-IC () ChIP ()

性能
供应商	CST
背景	Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
存放说明	-20C

参考图片

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (right).免疫组化分析石蜡包埋的人结肠腺癌。所用检测抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb #4858 (upper) (上图)浓度匹配的 Rabbit (DA1E) mAb IgG XP ® 同型对照(下图)。

Flow cytometric analysis of K-562 cells, untreated (green) or STI-treated (blue), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).流式细胞术分析K-562细胞，蓝色为STI处理组，绿色为未处理组，所用检测抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 与浓度匹配Rabbit (DA1E) mAb IgG XP ® 同型对照(红色)作比较。

Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测石蜡包埋的人结肠腺癌细胞，所用抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb #4858 (绿色，左图)与浓度匹配的 Rabbit (DA1E) mAb IgG XP ®同型对照(绿色，右图)作比较。蓝色伪彩为DNA荧光染料（产品信息为DRAQ5®#4084 ）。

Confocal immunofluorescent analysis of A549 cells using β-Tubulin (9F3) Rabbit mAb #2128 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测A549细胞，所用抗体为g β-Tubulin (9F3) Rabbit mAb #2128 (绿色，左图)与浓度匹配的 Rabbit (DA1E) mAb IgG XP ® 同型对照(绿色，右图)作比较。蓝色伪彩为DNA荧光染料（产品信息为DRAQ5®#4084 ）。

Confocal immunofluorescent analysis of normal rat cerebellum using DARPP-32 (19A3) Rabbit mAb #2306 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测正常大鼠小脑，所用检测抗体为DARPP-32 (19A3) Rabbit mAb #2306 (绿色，上图) 与浓度匹配的 Rabbit (DA1E) mAb IgG XP ®同型对照 (绿色，下图)作比较。蓝色伪彩为DNA荧光染料（产品信息DRAQ5®#4084 ）。

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The relative abundance of each DNA sequence enriched by Rabbit (DA1E) mAb IgG XP® Isotype Control (red) is compared to the amount of the same DNA sequence enriched by the histone H3-specific immunoprecipitations (blue).采用HeLa细胞已消化的染色质和已知抗体进行染色质免疫沉淀。实时定量PCR检测纯化DNA，所用引物分别为SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, 和SimpleChIP ® Human MYT-1 Exon 1 Primers #4493.每一条DNA序列的相对含量，所用抗体为Rabbit (DA1E) mAb IgG XP ®同型对照(红色) 与同一条DNA序列的含量做比较，后者通过为组蛋白H3特异的免疫沉淀检测（蓝色）。