| 货号 | 3438T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb detects PDK1 only when phosphorylated at Ser241. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.Several serine sites (Ser25, Ser241, Ser393/396 and Ser410) are phosphorylated on PDK1 in unstimulated human embryo kidney 293 cells, as well as IGF-1 stimulated cells (4). Phosphorylation on the activation loop Ser241 by autophosphorylation is necessary for PDK1 activity (4). |
| 存放说明 | -20C |
| 计算分子量 | 58 to 68 |
| 参考文献 | 1 . Belham, C. et al. (1999) Curr. Biol. 9, R93-R96. 2 . Toker, A. and Newton, A.C. (2000) Cell 103, 185-188. 3 . Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448. 4 . Casamayor, A. et al. (1999) Biochem J 342 (Pt 2), 287-92. |
Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD). Western blot分析PC3、 HCT116 wild-type 和 HCT116 PDK1 -/- cells 细胞提取物，所用抗体为Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 兔单抗(上) 和 Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691 (下). (HCT116 wild-type和HCT116 PDK1 -/- 细胞 由Johns Hopkins University, Baltimore, MD的Dr. Bert Vogelstein友情提供)。 | |
Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower). Western blot分析PC3 细胞提取物，未处理组和λ磷酸酶处理组， 所用抗体为Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb 兔单抗(上), PDK1 Antibody #3062 (中) 和 Akt Antibody #9272 (下)。 |
