| 货号 | 2859T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-IκBα (Ser32) (14D4) Rabbit mAb detects endogenous levels of IκBα only when phosphorylated at Ser32. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. 2 . Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. 3 . Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. 4 . Brown, K. et al. (1995) Science 267, 1485-8. 5 . Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 8 . Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63. |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-α (#2169, 20 ng/ml) for 5 minutes, using Phospho-IκBα (Ser32) (14D4) Rabbit mAb (upper), or IκBα (44D4) Rabbit mAb #4812 (lower). |
