Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb

货号： 2827T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	2827T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, F , IF-IC
目标/特异性	Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. The antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.
使用方法	WB(1:1000) F (1:800) IF-IC (1:200)

性能
供应商	CST
灵敏度	Endogenous
背景	Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).
存放说明	-20C
计算分子量	28
参考文献	1 . Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11. 2 . Zhu, L. et al. (1999) J Biol Chem 274, 33267-73. 3 . Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42. 4 . Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78. 5 . Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91. 6 . Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32. 7 . Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

参考图片

Confocal immunofluorescent analysis of SH-SY5Y cells, untreated, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析SH-SY5Y 细胞（未经处理），所用抗体为Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5^® #4084 (DNA荧光染料)。

Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content).

流式细胞仪分析 Jurkat 细胞，所用抗体为Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗，与碘化（DNA 含量）做比对。

Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1 μM, overnight) and with or without λ phosphatase, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (upper) or Bcl-2 #2876 (lower).

Western blot 免疫印迹检测分别经paclitaxel (1 μM, 过夜) 处理或未处理和经λ磷酸酶处理或未处理的Jurkat细胞的细胞提取液，所用抗体是Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗(上图) 或者 Bcl-2 #2876 (下图) 。