Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb【科瑞奥博】

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Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb

货号： 7237T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	7237T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IP
目标/特异性	Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
存放说明	-20C
计算分子量	37
参考文献	1 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3 . Liu, X. et al. (1996) Cell 86, 147-157. 4 . Li, P. et al. (1997) Cell 91, 479-489. 5 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9 . Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

参考图片

Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) or etoposide (25 μM, overnight), using Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb (upper) or total Caspase-9 Antibody (Human Specific) #9502 (lower).

Western免疫印迹。用Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 兔单抗(上图), 或者total Caspase-9 Antibody (Human Specific) #9502 (下图)研究未经处理或经 Staurosporine #9953 (1 μM, 3 hr) 或者etoposide (25 μM, 过夜)处理的HeLa 和Jurkat 细胞的细胞提取液。