| 货号 | 3073T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb detects endogenous levels of c-Kit only when phosphorylated at Tyr703. This antibody may cross-react with other tyrosine-phosphorylated RTKs. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
| 参考文献 | 1 . Martin, F.H. et al. (1990) Cell 63, 203-11. 2 . Yarden, Y. et al. (1987) EMBO J 6, 3341-51. 3 . Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25. 4 . Sattler, M. et al. (1997) J Biol Chem 272, 10248-53. 5 . Nocka, K. et al. (1990) EMBO J 9, 1805-13. 6 . Hirota, S. et al. (1998) Science 279, 577-80. 7 . Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62. 8 . Thömmes, K. et al. (1999) Biochem J 341 ( Pt 1), 211-6. 9 . Duensing, A. et al. (2004) Oncogene 23, 3999-4006. |
Western blot analysis of extracts of H526 cells, untreated or treated with SCF, using Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb (upper) or c-Kit Antibody #3392 (lower).用Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb（上）和c-Kit Antibody #3392（下）对经过和未经SCF刺激的H526细胞的提取物进行免疫印迹检测。 |
