| 货号 | 3133T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-Met (Tyr1349) (130H2) Rabbit mAb detects endogenous levels of Met only when phosphorylated at tyrosine 1349. This antibody may cross-react with other activated protein tyrosine kinases. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
| 参考文献 | 1 . Cooper, C.S. et al. Nature 311, 29-33. 2 . Bottaro, D.P. et al. (1991) Science 251, 802-4. 3 . Bardelli, A. et al. (1997) Oncogene 15, 3103-11. 4 . Taher, T.E. et al. (2002) J Immunol 169, 3793-800. 5 . Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32. 6 . Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14. 7 . Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118. |
Western blot analysis of cell lysates of H4IIE cells untreated or treated with HGF, using Phospho-Met (Tyr1349) (130H2) Rabbit mAb (upper), or Met (25H2) Mouse mAb #3127 (lower).用抗体Phospho-Met (Tyr1349) (130H2) Rabbit mAb (上)或Met (25H2) Mouse mAb #3127 (下)对未经处理的或HGF处理的H4IIE 细胞的提取物进行免疫印迹检测。 |
